Reporter

Part:BBa_K2176000:Design

Designed by: Miranda Halle   Group: iGEM16_UChicago   (2016-07-25)


yeGFP Reporter for LexA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 1870
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal SpeI site found at 1871
    Illegal PstI site found at 1885
    Illegal NotI site found at 12
    Illegal NotI site found at 1878
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal XhoI site found at 176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 1871
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6
    Illegal XbaI site found at 21
    Illegal SpeI site found at 1871
    Illegal PstI site found at 1885
    Illegal AgeI site found at 1865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1074


Design Notes

The GFP used in this construct was optimized for yeast before synthesis. Additionally, a tag was added at the end to make degradation faster.

The number of repeats of the LexA operator system was decided based on a previous iGEM part.


Source

The entire sequence for this part was synthesized as G-blocks and assembled via Gibson Assembly.

References